非规范氨基酸工具及其在膜蛋白研究中的应用

e Coin，托马斯·P·萨克玛

A new publication by Professor Irene Coin from Leipzig University provides an overview of the development of genetic code expansion (GCE) technology and its application to membrane proteins. The article, co-authored by Professor Thomas Sakmar (Rockefeller University, New York), is published in the renowned journal Chemical Reviews.

莱比锡大学 Irene Coin 教授的新出版物概述了遗传密码扩展 (GCE) 技术的发展及其在膜蛋白中的应用。该文章由纽约洛克菲勒大学 Thomas Sakmar 教授与人合着，发表在著名期刊《Chemical Reviews》上。

Moreover, Professor Coin's lab has been honoured by the non-profit organisation Addgene with its Blue Flame Award.

此外，Coin教授的实验室还获得了非盈利组织Addgene颁发的蓝色火焰奖。

“All proteins in our body are made up of twenty building blocks called canonical amino acids. Our genetic code determines which amino acids are incorporated. By expanding the genetic code, new building blocks can be artificially inserted into proteins,” says Professor Irene Coin.

“我们体内的所有蛋白质都是由二十种称为经典氨基酸的构建块组成。我们的遗传密码决定了哪些氨基酸被纳入。通过扩展遗传密码，新的构建模块可以人工插入蛋白质中，”艾琳·科因教授说。

"This allows us to produce proteins that do not occur in nature. This technique is primarily used to develop novel protein therapeutics and to better understand the structure and function of natural proteins."

“这使我们能够生产自然界中不存在的蛋白质。这项技术主要用于开发新型蛋白质疗法并更好地了解天然蛋白质的结构和功能。”

Coin and her colleague have now published a comprehensive overview of the historical development of GCE technology and its application to membrane proteins in Chemical Reviews, based on around 450 citations.

Coin 和她的同事现在在《Chemical Reviews》上发表了 GCE 技术的历史发展及其在膜蛋白应用的全面概述，引用了大约 450 次。

“Crucially, GCE makes it possible to obtain information about these proteins directly from living cells – information that is inaccessible using conventional biophysical methods.”

“至关重要的是，GCE 使得直接从活细胞获取有关这些蛋白质的信息成为可能——这是使用传统生物物理方法无法获取的信息。”

The overview is structured according to the potential applications of the technology.

概述是根据该技术的潜在应用来构建的。

“This makes it easier for non-specialist researchers to understand and use the technique and supports its practical implementation,” says the professor. “We are also working in the laboratory to develop ever more efficient systems for the general application of GCE.”

“这使得非专业研究人员更容易理解和使用该技术并支持其实际实施，”教授说。 “我们还在实验室致力于开发更高效的系统，以实现 GCE 的普遍应用。”

The Leipzig biochemist's laboratory recently received the Blue Flame Award from the non-profit organisation Addgene. Since 2016, Addgene has awarded this prize to researchers who have deposited at least one plasmid that has been requested more than 100 times. The plasmid in question was developed as part of Dr Robert Serfling's doctoral thesis.

莱比锡生物化学家的实验室最近获得了非营利组织 Addgene 颁发的蓝色火焰奖。自 2016 年以来，Addgene 将这一奖项授予至少保藏了一个已被请求超过 100 次的质粒的研究人员。所讨论的质粒是作为罗伯特·瑟夫林博士博士论文的一部分而开发的。

Plasmids are small, ring-shaped pieces of DNA that allow desired genes to be imported into cells. Addgene is a plasmid repository that facilitates the exchange of genetic material between laboratories worldwide.

质粒是小的环形 DNA 片段，可以将所需的基因导入细胞中。 Addgene 是一个质粒存储库，可促进全球实验室之间遗传物质的交换。

With her work on genetic code expansion, Irene Coin is making an important contribution to leading research networks at Leipzig University, in particular Collaborative Research Centre (CRC) 1423, "Structural Dynamics of GPCR Activation and Signaling".

Irene Coin 凭借其在遗传密码扩展方面的工作，为莱比锡大学的领先研究网络做出了重要贡献，特别是合作研究中心 (CRC) 1423“GPCR 激活和信号传导的结构动力学”。