非規範胺基酸工具及其在膜蛋白研究中的應用

e Coin，托馬斯·P·薩克瑪

A new publication by Professor Irene Coin from Leipzig University provides an overview of the development of genetic code expansion (GCE) technology and its application to membrane proteins. The article, co-authored by Professor Thomas Sakmar (Rockefeller University, New York), is published in the renowned journal Chemical Reviews.

萊比錫大學 Irene Coin 教授的新出版物概述了遺傳密碼擴展 (GCE) 技術的發展及其在膜蛋白中的應用。這篇文章由紐約洛克斐勒大學 Thomas Sakmar 教授與人合著，發表在著名期刊《Chemical Reviews》。

Moreover, Professor Coin's lab has been honoured by the non-profit organisation Addgene with its Blue Flame Award.

此外，Coin教授的實驗室也獲得了非營利組織Addgene頒發的藍色火焰獎。

“All proteins in our body are made up of twenty building blocks called canonical amino acids. Our genetic code determines which amino acids are incorporated. By expanding the genetic code, new building blocks can be artificially inserted into proteins,” says Professor Irene Coin.

「我們體內的所有蛋白質都是由二十種稱為經典胺基酸的構建塊組成。我們的遺傳密碼決定了哪些胺基酸被納入。透過擴展遺傳密碼，新的建造模組可以人工插入蛋白質中，」艾琳·科因教授說。

"This allows us to produce proteins that do not occur in nature. This technique is primarily used to develop novel protein therapeutics and to better understand the structure and function of natural proteins."

“這使我們能夠生產自然界中不存在的蛋白質。這項技術主要用於開發新型蛋白質療法並更好地了解天然蛋白質的結構和功能。”

Coin and her colleague have now published a comprehensive overview of the historical development of GCE technology and its application to membrane proteins in Chemical Reviews, based on around 450 citations.

Coin 和她的同事現在在《Chemical Reviews》上發表了 GCE 技術的歷史發展及其在膜蛋白應用的全面概述，引用了大約 450 次。

“Crucially, GCE makes it possible to obtain information about these proteins directly from living cells – information that is inaccessible using conventional biophysical methods.”

“至關重要的是，GCE 使得直接從活細胞獲取有關這些蛋白質的信息成為可能——這是使用傳統生物物理方法無法獲取的信息。”

The overview is structured according to the potential applications of the technology.

概述是根據該技術的潛在應用來建構的。

“This makes it easier for non-specialist researchers to understand and use the technique and supports its practical implementation,” says the professor. “We are also working in the laboratory to develop ever more efficient systems for the general application of GCE.”

「這使得非專業研究人員更容易理解和使用該技術並支持其實際實施，」教授說。 “我們也致力於實驗室開發更高效的系統，以實現 GCE 的普遍應用。”

The Leipzig biochemist's laboratory recently received the Blue Flame Award from the non-profit organisation Addgene. Since 2016, Addgene has awarded this prize to researchers who have deposited at least one plasmid that has been requested more than 100 times. The plasmid in question was developed as part of Dr Robert Serfling's doctoral thesis.

萊比錫生物化學家的實驗室最近獲得了非營利組織 Addgene 頒發的藍色火焰獎。自 2016 年以來，Addgene 將這項獎項授予至少保藏了一個已被要求超過 100 次的質粒的研究人員。所討論的質粒是作為羅伯特·瑟夫林博士博士論文的一部分而開發的。

Plasmids are small, ring-shaped pieces of DNA that allow desired genes to be imported into cells. Addgene is a plasmid repository that facilitates the exchange of genetic material between laboratories worldwide.

質粒是小的環形 DNA 片段，可以將所需的基因導入細胞中。 Addgene 是一個質粒儲存庫，可促進全球實驗室之間遺傳物質的交換。

With her work on genetic code expansion, Irene Coin is making an important contribution to leading research networks at Leipzig University, in particular Collaborative Research Centre (CRC) 1423, "Structural Dynamics of GPCR Activation and Signaling".

Irene Coin 憑藉其在遺傳密碼擴展方面的工作，為萊比錫大學的領先研究網絡做出了重要貢獻，特別是合作研究中心 (CRC) 1423「GPCR 活化和訊號傳導的結構動力學」。